Western Blot:Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.
Article Snippet: Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.. The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).. After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA, USA) at 1:1000 dilution in PBS for 90 mins, respectively.After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA,..
Expressing:Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.
Article Snippet: Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.. The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).. After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA, USA) at 1:1000 dilution in PBS for 90 mins, respectively.After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA,..
Membrane:Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.
Article Snippet: Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.. The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).. After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA, USA) at 1:1000 dilution in PBS for 90 mins, respectively.After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA,..
Incubation:Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.
Article Snippet: Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.. The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).. After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA, USA) at 1:1000 dilution in PBS for 90 mins, respectively.After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA,..
Immunostaining:Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.
Article Snippet: Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.. The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).. After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA, USA) at 1:1000 dilution in PBS for 90 mins, respectively.After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA,..
Microscopy:Article Title: Induction of Parkinson disease-related proteins in motor neurons after transient spinal cord ischemia in rabbits.
Article Snippet: Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.Then they were incubated with primary antibodies at 1:1000 dilution for 20 h at 41C.. The primary antibodies used were as follows mouse monoclonal anti-DJ-1 antibody (SC55572; Santa Cruz Biotechnology, Inc., California, USA), goat polyclonal anti-PINK1 antibody (SC-32584; Santa Cruz Biotechnology, Inc., California, CA, USA), and mouse monoclonal anti-a-Syn antibody (SC-12767; Santa Cruz Biotechnology, Inc., California, USA).. After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA, USA) at 1:1000 dilution in PBS for 90 mins, respectively.After washing in phosphate-buffered saline (PBS), the membranes were incubated with alkaline phosphtase-conjungate anti-goat IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7107, Invitrogen, CA, USA) and alkaline phosphtase-conjungate anti-mouse IgG (Western Breeze Chromogenic Western Blot immunodetection kit, WB 7103, Invitrogen, CA,..
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